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论文 |
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论文题目: |
Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants
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论文题目英文: |
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作者: |
Huang, Junchao; Zhong, Yujuan; Sandmann, Gerhard; Liu, Jin; Chen, Feng
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论文出处: |
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年: |
2012
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卷: |
236 |
期: |
2 |
页: |
1390 |
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发表期刊: |
PLANTA |
第一作者所在部门: |
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收录类别: |
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影响因子: |
3.088
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摘要: |
beta-Carotene ketolase (BKT) catalyzes the rate-limiting steps for the biosynthesis of astaxanthin. Several bkt genes have been isolated and explored to modify plant carotenoids to astaxanthin with limited success. In this study, five algal BKT cDNAs were isolated and characterized for the engineering of high-yield astaxanthin in plants. The products of the cDNAs showed high similarity in sequence and enzymatic activity of converting beta-carotene into canthaxanthin. However, the enzymes exhibited extremely different activities in converting zeaxanthin into astaxanthin. Chlamydomonas reinhardtii BKT showed the highest conversion rate (ca 85 %), whereas, Neochloris wimmeri BKT exhibited very poor activity of ketolating zeaxanthin. Expression of C. reinhardtii BKT in tobacco led to a twofold increase of total carotenoids in the leaves with astaxanthin being the predominant. The bkt genes described here provide a valuable resource for metabolic engineering of plants as cell factories for astaxanthin production. |
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