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论文 |
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论文题目: |
SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY
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论文题目英文: |
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作者: |
Ping, Li; Wang Yeling; Guo Zhiyong; Wu, Shibiao; Kennelly, Edward J.; Wang Jianjun; Li, Tang; Long Chunlin
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论文出处: |
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年: |
2014
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卷: |
37 |
期: |
18 |
页: |
2598-2609 |
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发表期刊: |
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES |
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摘要: |
Under LC-TOF-MS fraction-guided analysis, the n-butanol was extracted from Bidens pilosa. Three bioactivity isomers compounds, including methyl 3,4-dicaffeoyl quinate (1), methyl 3,5-dicaffeoyl quinate (2), and methyl 4,5-dicaffeoyl quinate (3) were successfully isolated by countercurrent chromatography (CCC) with two phase solvent system composed of chloroform-methanol-water-n-buthanol (4:3:2:0.5, v/v) elution. Their purities were 93.7%, 82.4%, and 91.8% determined by HPLC. HR-ESI-MS and NMR was used to confirm their structures. This is the first time the isolation of geometrical isomers of methyl dicaffeoyl quinates by CCC has been reported. |
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